目的：建立甲基苯丙胺(methamphetamine，MA)和苯丙胺(amphetamine，AMP)的反相高效液相色谱测定方法。

　　方法：采用C18柱，以甲醇-磷酸盐缓冲液为流动相，流速1.0 mL/min，检测波长为215 nm，同时收集190～360 nm的紫外光谱图，并以此与保留时间作为定性依据。在定性的基础上，建立定量检测方法，并对方法进行评价研究。

　　结果：所建方法能良好分离MA和AMP，结合判断依据能准确定性；MA在1.4～270 μg/mL浓度范围内线性关系良好，R2=1，日内标准偏差(RSD)与日间RSD均＜2.4%，检出限为0.73 μg/mL，平均加样回收率为102.5%；AMP在0.9～580 μg/mL浓度范围内线性关系良好，R2=0.9999，日内RSD与日间RSD均＜2.3%，检出限为0.52 μg/mL，平均加样回收率为101.7%。

　　结论：此方法简便、快速、准确，适用于MA与AMP的检测。

　　关键词：高效液相色谱法；甲基苯丙胺；苯丙胺

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